The MIC Study of Antibiotics Used in the Treatment of Children with Urinary Tract Infections caused by E.coli using E-test and ts Comparison with Disk Diffusion

Background and Objective: Urinary tract infection (UTI) is one of the most common bacterial infections in the childhood which could result in chronic renal failure and hypertension. Antibiotic resistance is increasing due to widely using of antibiotics. The aim of this study was to determine the MIC of antibiotics which are using in the treatment of UTI in children by E-test. Materials and Methods: In this descriptive study, ۸۷ E.coli strains were isolated from the urine samples of the patients with UTI. E.coli antimicrobial susceptibility was determined using E-test. The MIC for each antibiotic was determined and classified using NCCLS criteria. Results: Eighty seven urine samples were collected from ۵۷ girls (۶۵.۵%) and ۳۰ boys (۳۴.۵%). The mean age for girls was ۶۱ months and for boys was ۴۱ months (p=۰.۰۱۵). The MIC (۵۰ and ۹۰ percentiles) for each antibiotic was as fallow: Ampicillin (۲۵۶, ۲۵۶), Amikacin (۱/۵, ۴/۸), Gentamycin (۰.۳۸, ۳۲), Nalidixic Acid (۱/۵, ۲۵۶), Ceftriaxon (۰.۰۲۳, ۳۲), Cefixim (۰.۱۹, ۲۵۶) and Trimethoprim-Sulfamethoxazole (۳۲, ۳۲). The antibiotic susceptibility rate for each antibiotic was as fallow: Ampicillin (۲۱.۸%), Amikacin (۹۲%), Gentamycin (۷۵.۵%), Nalidixic Acid (۶۴/۴%), Ceftriaxon (۷۲/۴%), Cefixim (۶۵/۵%) and Trimethoprim-Sulfamethoxazole (۴۱/۴%). Conclusion: This study showed that the antibiotic resistance of E.coli was very high. Due to increasing rate of E.coli resistance to Ampicillin and Cotrimoxasole in children, it is better to reconsider the empirical therapy with these antibiotics. Since in this study a lower resistance rate of E.coli was observed for Amikacin and Gentamycin therefore, we could suggest these antibiotics as alternatives in the treatment of children with urinary tract infection.

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Key words: E.coli , UTI , E-test , MIC