The Effect of Bone Marrow Derived Mesenchymal Stem Cells on the Survival of Random Skin Flap on Sterptozotocin-Induced Diabetic Rats

Background & Objective:  Wound dressing and healing in diabetic patients is encountered with many problems. This study aims to investigate the effect of bone marrow derived mesenchymal stem cells (BM-MSCs) on the survival of random skin flap (RSF) on Streptozotocin-induced diabetic rats (STZ) using an optical microscope. Materials & Methods:  In this study, ۶۰ male Albino Wistar rats were used (average weight ۲۵۰-۳۰۰ gr). The rats were divided into six groups: ۱). Health-Non (HN), ۲). Health- Cells (HC), ۳). Health –Sham (HS), ۴). Diabetic-Non (DN) that were became diabetic by injecting STZ ۷۰ mg/kg intraperitoneally), ۵). Diabetic-Sham (DS), ۶). And Diabetic-Cell (DC). In all groups, the day of surgery was considered as the zero day, on the back area of animal, the flap was created with a size of ۸ × ۳ cm and the BM-MSCs were performed. The sampling was performed on day ۷ after surgery from the region where Transitional Zone (TZ) necrosis was initiated. Results:  BM-MSCs increased the number of blood vessels (P=۰.۰۰۹) and the histology parameters (wound demarcation P=۰.۰۰۰۱, granulation tissue P=۰.۰۰۰۱) significantly compared to the control group. But this increase was not significant in the area of the survival region. Conclusion:  It was concluded that after treatment with BM-MSCs, the wound healing process in both non-diabetic and diabetic groups was increased in accordance with histological characteristics.

Keywords:

Diabetes mellitus , Random skin flap , Bone marrow , Stem cells , Streptozotocin , Survival