Cytotoxic Activities of Extracts of Garlic and Green Tea

Background and Aim: Allium sativum belongs to the Liliaceae family and Green Tea are widely used in folk medicine. Antibacterial, anticancer and antioxidant activity of these genuses have been previously reported. The current study, aimed at investigating the cytotoxic activity of the extracts from. sativum and Green Tea on breath cancer cell line (MCF-۷) and HDF Methods: We examined the cytotoxicity of different concentrations (۲۵۰,۵۰۰,۷۵۰ μg/ml) of Garlic and Green Tea extracts on MCF-۷ and HDF cell lines. The cell lines were grown in RPMI supplemented with ۱۰% FBS, ۱% penicillin and streptomycin. The cells were allowed to incubate at ۳۷ºC in an atmosphere that contained ۵% CO۲ and ۹۵% humidity. The standard MTT assay was performed to estimate cell viability after treatment of garlic and Green Tea extracts.Results: The results of the MTT assay showed that garlic extract had time-and concentration-dependent anticancer activities on the MCF- ۷ cell line (p<۰.۰۱). But, we didn’t show any effect of Green Tea extract on MCF-۷ and HDF cell lines.Conclusion: Our results, compared with results from other studies, have suggested that garlic extract has a good potential to control cancer cells

Keywords:

Garlic , Green Tea , Breast Cancer , Cytotoxicity