β-LAPachone is renoprotective in streptozotocin-induced diabetic mice via regulating the PI۳K/Akt/mTOR signaling pathway
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 24، Issue: 5
Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-24-5_012
تاریخ نمایه سازی: 18 اردیبهشت 1400
Abstract:
Objective(s): β-LAPachone (B-LAP) is a natural product with established anti-inflammatory properties. In this study, we investigated the protective potential of B-LAP against diabetic nephropathy in streptozotocin (STZ) induced diabetic mice.Materials and Methods: Diabetes induction in mice was carried out by a single intraperitoneal injection of STZ. ۲.۵ mg/kg/day and ۵ mg/kg/day doses of B-LAP were administered orally for twelve weeks and renal histoarchitecture, caspase-۳, tumor necrosis factor-alpha (TNF-α), malondialdehyde (MDA), glutathione peroxidase (GPX), as well as urinary nephrin and neutrophil gelatinase-associated lipocalin (NGAL) were evaluated. Additionally, kidney levels of PI۳K, phosphorylated (p)-Akt, p-mTOR, p-CREB, and SIRT۱ were assessed in the present investigation.Results: ۵ mg/kg B-LAP significantly decreased urinary excretions of nephrin and NGAL. It also mitigated renal TNF-α and MDA levels and simultaneously improved GPX activities. ۵ mg/kg B-LAP improved renal function in diabetic mice as indicated by elevated values of creatinine clearance. While B-LAP elevated renal levels of SIRT۱, it alleviated PI۳K, p-Akt, p-mTOR, and p-CREB levels in the kidneys of diabetic mice.Conclusion: Collectively, these findings suggest B-LAP as a potential renoprotective agent in STZ-induced diabetic mice probably via modulating the PI۳K/Akt/mTOR pathway.
Keywords:
Akt β , LAPachone Diabetic nephropathy mTOR PI۳K
Authors
Davoud Sanajou
Department of Biochemistry, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Saman Bahram Beigi
Department of Biochemistry, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Somayeh Aslani
Department of Biochemistry, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
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