Preliminary phytochemical, pharmacognostic and physicochemical evaluation of leaf of Gomphrena serrata

Background and aims: To analyze the pharmacognostic characteristics and physiochemical parameters of the leaves of Gomphrena serrata (G. serrata).Methods: Microscopic characters and powder analysis had been carried out with the help of a microscope. The physiochemical properties such as loss on drying, total ash value, acid insoluble ash value, water soluble ash value, extractive values and fluorescence of G. serrata had been performed.Results: Macroscopically, the leaves are simple, elliptical in shape, rounded or obtuse apex with an entire margin. Microscopically, the leaf showed the presence of epidermal cells with uniseriate multicellular covering trichomes and anomocytic stomata, followed by ۱-۲ layered collenchymatous cells and ۱۰-۱۵ numbered conjoint, collateral closed vascular bundles are some of the diagnostic characteristics observed from an anatomical study. Powder microscopy of leaf revealed the presence of uniseriate multicellular covering trichomes, lignified xylem vessels, epidermis with anomocytic stomata and parenchyma cells. The investigations also included leaf surface data i.e., quantitative leaf microscopy and fluorescence analysis. Physiochemical parameters such as loss on drying, extractive values and ash values were also determined. Preliminary phytochemical screening showed the presence of flavonoids, alkaloids, tannins, steroids, carbohydrates, glycosides, amino acids and proteins.Conclusion: The morphological, microscopical and physicochemical parameter results provided in this paper may be utilized as a basis for the preparation of a monograph on G. serrata leaves. 

Keywords:

Pharmacognostic , Gomphrena serrata , Anomocytic stomata , Lignified xylem vessels , Phytochemical and Physicochemical analysis