Kinetics of inhibitory effect of ethyl acetate leaf fraction of Lecaniodiscus cupanoides on enzymes linked to carbohydrate metabolism: An in vitro assessment
Publish place: Future Natural Products، Vol: 4، Issue: 2
Publish Year: 1397
نوع سند: مقاله ژورنالی
زبان: English
View: 268
This Paper With 15 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_JHPH-4-2_005
تاریخ نمایه سازی: 11 خرداد 1400
Abstract:
Background and aims: This study investigates the kinetics of inhibitory activities of ethyl acetate leaf fraction of Lecanodiscus cupanoides on 𝛼-amylase and 𝛼-glucosidase. Methods: This was done using 𝛼-amylase and 𝛼-glucosidase enzymes from Aspergillus oryzae and Saccharomyces cerevisiae respectively, active component and antioxidants capabilities of the fraction were also analyzed. Results: The fraction scavenged DPPH, OH*, and ABTS+ and chelated Fe۲+ with the IC۵۰ values of ۰.۴۶, ۰.۷۰, ۰.۷۵ and ۰.۹۷ mg/mL respectively. However, significant and mild inhibitory effect was also observed in 𝛼- amylase and glucosidase with IC۵۰ values (۰.۷۳ and ۰.۵۸ mg/mL) in concentration dependent pattern. Kinetic analysis of the fraction revealed an uncompetitive and non-competitive mode of inhibition for 𝛼-amylase and 𝛼-glucosidase respectively. Conclusion: This study suggests that ethylacetate fraction of this plant possesses antidiabetic property as a result of its ability to inhibit the metabolism of carbohydrate hydrolyzing enzymes. The elicited activities of the ethyl acetate fraction Lecanodiscus cupanoides may be due to the presence of phytochemicals of utmost pharmacological importance present in the fraction.
Keywords:
Authors
Abdulwakeel Ajao
Department of Botany, Obafemi Awolowo University, Ile-Ife, Nigeria .
Rasheed Adigun
Department of Pure and Applied chemistry, Usmanu Danfodiyo University Sokoto, Nigeria.
Abdullahi Jimoh
Department of Microbiology, University of Kwazulu Natal, Westville Campus, South Africa
Yusuf Adams
Department of Botany, Obafemi Awolowo University, Ile-Ife, Nigeria ۲۲۰۰۰۵,
Muideen Sanni
Department of Chemistry, Obafemi Awolowo University, Ile-Ife, Nigeria ۲۲۰۰۰۵,
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :