Evaluation of the cytotoxic effects of cisplatin-loaded magnetic nanoparticles on CT۲۶ cell line

CDDP is one of the most potent antitumor agents; however, it is accompanied by severe side effects, including digestive tract reactions, renal toxicity, bone marrow suppression, and neurotoxicity. Furthermore, long-term use of CDDP can induce drug resistance. In recent years, much attention has been paid to developing targeted drug delivery systems, which are a promising strategy for increasing therapeutic drug accumulation in cancer cells, while reducing toxicity to normal tissues. Among these, magnetic nanoparticles are attracting more interest. Hence, in the present study, we employed Fe۳O۴ hydroxyapatite nanoparticles to improve the therapeutic effect of cisplatin (CDDP) in colorectal cancer. So, magnetic hydroxyapatite nanoparticle (mHAP) was fabricated and CDDP was loaded in porous of hydroxyapatite. The synthesized nanocomposite was characterized by a UV–Visible spectrophotometer, FT-IR, and VSM. In vitro, biological experiments revealed that a high efficiency in the cytotoxic effect of Cs.CDDP:mHAP in CT۲۶ colorectal cancer, using MTT assay and Ao/EtBr staining. The IC۵۰ value of treated cells with CDDP (۲.۸۴±۰.۱ μg/ml) was decreased to be ۲.۳۴±۰.۰۹۲ μg/ml when the cells were treated with Cs.CDDP:mHAP. A pronounced increase in apoptosis was observed in the cells treated with the nanocomposite as compared with that in the cells treated with the CDDP, which is in accordance with the MTT assay results.

Keywords:

Drug delivery system , Magnetic hydroxyapatite nanoparticles , Cisplatin , CT۲۶ cells