Identification of Leishmania Species Causing Cutaneous Leishmaniasis Using Random Amplified Polymorphic DNA (RAPD-PCR) in Kharve, Iran
Publish Year: 1392
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_RBMB-1-2_004
تاریخ نمایه سازی: 10 شهریور 1400
Abstract:
Background: Leishmaniasis, especially cutaneous leishmaniasis, is considered an important health problem in many parts of Iran including Kharve, Khorasan Razavi province. Cutaneous leishmaniasis is caused by various species of Leishmania, each having a different secondary host. Thus, identifying the parasites’ specie is of paramount importance for containment strategy planning. The morphological differentiation of Leishmania species is not possible, rendering the molecular methods as the sole means to this purpose. Therefore, to identify the causative agent of cutaneous leishmaniasis in Kharve, Random Amplified Polymorphic DNA-PCR (RAPD-PCR) was used.
Methods: The disease was first confirmed by direct smears. Samples were gathered from ۲۲ patients with established cutaneous leishmaniasis. The samples were immediately cultured in NNN medium, followed by sub-culture in RPMI-۱۶۴۰. Afterwards, DNA was extracted and amplified using RAPD-PCR. Electrophoresis patterns from each isolate were compared with reference strains of Leishmania major (L. major) and Leishmania tropica (L. tropica).
Results: The results of this study indicated that the parasite causing cutaneous leishmaniasis in Kharve is L. tropica.
Conclusion: It seems that L. tropica is the only causative agent of cutaneous leishmaniasis in Kharve, and RAPD-PCR is a suitable tool for Leishmania characterization in epidemiological studies.
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Authors
Fatemeh Saadabadi
Parasitology & Medical Mycology Department, School of Medicine, Mashhad University of Medical Sciences Mashhad, Iran
Masoud Mohajery
Parasitology & Medical Mycology Department, School of Medicine, Mashhad University of Medical Sciences Mashhad, Iran
Elham Poostchi
Parasitology & Medical Mycology Department, School of Medicine, Mashhad University of Medical Sciences Mashhad, Iran
Seyyed Ali Akbar Shamsian
Parasitology & Medical Mycology Department, School of Medicine, Mashhad University of Medical Sciences Mashhad, Iran