Nicotine-induced damages in testicular tissue of rats; evidences for bcl-۲, p۵۳ and caspase-۳ expression
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 20، Issue: 2
Publish Year: 1396
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-20-2_014
تاریخ نمایه سازی: 28 مهر 1400
Abstract:
Objective(s): Present study was performed in order to uncover new aspects for nicotine-induced damages on spermatogenesis cell lineage. Materials and Methods: For this purpose, ۳۶ mature male Wistar rats were divided into three groups as; control-sham (۰.۲ ml, saline normal, IP), low dose (۰.۲ mg/kg BW-۱, IP) nicotine-received and high dose (۰.۴ mg/kg BW-۱, IP) nicotine-received groups. Following ۷ weeks, the expression of bcl-۲, p۵۳ and caspase-۳ at mRNA and protein levels were investigated by using reverse-transcriptase PCR (RT-PCR) and immunohistochemical (IHC) analyses, respectively. Moreover, the serum level of FSH, LH and testosterone were evaluated. Finally, the mRNA damage was analyzed by using special fluorescent staining. Results: Nicotine, at both dose levels, decreased tubular differentiation, spermiogenesis and repopulation indices and enhanced cellular depletion. Animals in nicotine-received groups exhibited a significant (P<۰.۰۵) reduction at mRNA and protein levels of bcl-۲. More analyses revealed a remarkable (P<۰.۰۵) enhancement in expression of p۵۳ and caspase-۳ in comparison to control-sham animals. Finally, nicotine resulted in a significant (P<۰.۰۵) reduction in serum level of testosterone and elevated mRNA damage. Conclusion: Our data showed that, nicotine by suppressing the testosterone biosynthesis, reducing mRNA and protein levels of bcl-۲ and up regulating the p۵۳ and caspase-۳ mRNA and protein levels adversely affects the spermatogenesis and results in cellular depletion.
Authors
Maryam Mosadegh
Department of Comparative Histology & Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Shapour Hasanzadeh
Department of Comparative Histology & Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Mazdak Razi
Department of Comparative Histology & Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
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