The Simultaneous Effect of Linum Usitatissimum Supplementation and Aerobic Training on ۶-Methylguanine and ATP in the Endothelial Aorta and Heart Tissues in Rats Poisoned with H۲O۲

Background and Objective: As a high-energy demanding tissue, the heart is exposed to a high level of ROS molecules such as H۲O۲, leading to cardiovascular disorders through damaging macromolecules such as DNA and disrupting ATP production. Hence, this study aimed to investigate the simultaneous effect of aerobic exercise (Ae) and Linum Usitatissimum (Lu) supplementation on DNA damage and ATP synthesis in heart and aorta endothelial tissues in rats poisoned with hydrogen peroxide (H۲O۲). Material and Methods: ۵۶ male Wistar Albino rats were randomly divided into ۷ groups, including HC (Healthy Control), TC (Toxicated Control), Lu۱ (Received ۵ mg/kg of Lu), Lu۲ (Received ۱۰ mg/kg of Lu), Ae (Received only Aerobic Exercise), Ae Lu۱, and Ae Lu۲. Then, all groups got poisoned by H۲O۲ except HC. Next, they received Linum Usitatissimum (Lu) supplementation and Low-Intensity Interval Training (LIIT). Finally, ۲۴h after the last treatment session, the level of ۶-methylguanine (۶MG) and ATP were measured via the ELISA technique in cardiovascular tissue. Results: The findings determined that Lu supplementation and Ae significantly diminish the ۶-methyl guanine level in endothelial (F=۱۱۱.۳, p=۰.۰۰۰۸, ƞ=۰.۹۸۲۳) and heart cells (F=۱۴۷.۹, p=۰.۰۰۰۵, ƞ=۰.۹۸۶۷). Also, the ATP level was increased significantly in endothelial (F=۳۴۲.۶, p=۰.۰۰۰۳, ƞ=۰.۹۹۴۲) and heart cells (F=۱۳۵.۱, p=۰.۰۰۱۳, ƞ=۰.۹۸۵۴). However, no considerable changes were found for both factors in groups who received Ae or Lu singularly. Conclusion: The study showed that concurrent administration of Lu and Ae could exert dynamic cardioprotective properties through their antioxidant effects.

Keywords:

H۲O۲ Toxicity , Mitochondrial , Cardiovascular Diseases , Herbal Medicine , Rehabilitation , Exercise