Biofilm Formation and Detection of IcaAB Genes in Clinical Isolates of Methicillin Resistant Staphylococcus aureus
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 14، Issue: 2
Publish Year: 1390
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-14-2_005
تاریخ نمایه سازی: 3 آبان 1400
Abstract:
Objective(s) Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of nosocomial and community infections. Biofilm formation, mediated by a polysaccharide intercellular adhesin (PIA) and encoded by the ica operon, is considered to be an important virulence factor in both S. epidermidis and S. aureus. However, the clinical impact of the ica locus and PIA production is less well described in S. aureus. We studied biofilm formation in clinical isolates of MRSA in relation to the presence of the ica operon. Materials and Methods Forty five MRSA were studied for biofilm formation by colony morphology on Congo red agar (CRA) and the microtitre plate assay (MtP). Presence of the ica genes was detected by PCR and specific primers. Results The results showed that ۵۳.۳% of the isolates had the potential to form biofilm by colony morphology of which, ۷۵% carried the ica operon. Weak biofilm production was observed in the MtP assay by ۵۷.۸%, of which ۵۳.۸% harbored the ica operon. However, about ۷۰% of biofilm non-producers also carried the ica operon. Conclusion Overall, there was no agreement between the icaAB gene carriage and biofilm phenotype by either of the two phenotypic methods. However, ۹۱% of biofilm formers on CRA also produced biofilm in the MtP assay.
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Authors
Fereshteh Eftekhar
Microbiology Department, Faculty of Biological Sciences, Shahid Beheshti University, G.C., Evin, Tehran, Iran
Taraneh Dadaei
Microbiology Department, Faculty of Biological Sciences, Shahid Beheshti University, G.C., Evin, Tehran, Iran
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