Effects of Multivitamins and Known Teratogens on Chick Cardiomyocytes Micromass Culture Assay
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 16، Issue: 9
Publish Year: 1392
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-16-9_008
تاریخ نمایه سازی: 4 آبان 1400
Abstract:
Objective(s): This study aimed to find out whether the chick cardiomyocyte micromass (MM) system could be employed to predict the teratogenecity of common environmental factors. Different multivitamins and over the counter drugs were used in this study. Materials and Methods: White Leghorn ۵-day-old embryo hearts were dissected and trypsinized to produce a cardiomyocyte cell suspension in Dulbecco's Modified Eagle's Medium. The cultures were incubated at ۳۷۰C in ۵% CO۲ in air, and observations were made at ۲۴, ۴۸ and ۱۴۴ hr, for the detection of cell beating. Cellular viability was assessed using the resazurin assay and cell protein content was assessed by the kenacid blue assay. It was observed that while not affecting total cell number folic acid, vitamin C, sodium fluoride and ginseng did not significantly reduced cell activity and beating. However cadmium chloride significantly reduced the beating, cell viability and cell protein content in micromass cultures. Results: The results demonstrate the potential of the chick cardiomyocyte MM culture assay to identify teratogens/embryotoxins that alter morphology and function, which may result in either teratogenic outcome or cytotoxicity. Conclusion: This could form part of a screen for developmental toxicity related to cardiac function
Keywords:
Chick cardiomyocyte Environmental teratogens Micromass culture Multivitamins
Authors
Samreen Memon
Department of Anatomy Liaquat University of Medical Sciences, Pakistan
Margaret Pratten
School of Biomedical Sciences, University of Nottingham, UK
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