Effect of Germinated Black Rice Krisna Extract on Fasting Blood Glucose and Body Weight in Diabetes Mellitus Rats

Background: Diabetes mellitus (DM) is a non-communicable disease that is generally associated with fasting blood glucose (FBG) level of >۱۲۶ mg/dL and weight loss. Germinated black rice krisna (GBRK) extract contains phytochemicals with antioxidant and anti-diabetic properties. The purpose of this study was to assess the impact of GBRK extract on FBG level and body weight (BW) in rat model of DM.Methods: In an in vivo pre- and post-test study ۳۶ male Wistar rats with BW design, of ۱۵۰-۲۰۰ g were enrolled and divided into ۶ groups, including control (C), negative control (NC), GBRK ۵۳۵ mg/kg BW (P۱), GBRK ۱۰۷۰ mg/kg. BW (P۲), and GBRK ۲۱۴۰ mg/kg BW (P۳). Diabetes was induced using streptozotocin (STZ) and nicotinamide (NA) and the intervention period was ۱۴ days. Measurement of FBG level was by quantitative enzymatic colorimetric test of Glucose Oxidase Phenol ۴-Aminoantipyrine (GOD-PAP) and body weight was determined by a digital scale.Results: The administration of GBRK extract in P۱, P۲, and P۳ groups could significantly reduce the FBG (p <۰.۰۱). GBRK in P۱, P۲, and P۳ groups could also significantly improve the body weight to normal state too (p <۰.۰۱).Conclusion: GBRK extract at doses of ۵۳۵, ۱۰۷۰, and ۲۱۴۰ mg/kg BW for ۱۴ days was demonstrated to improve metabolic disorders in diabetes by reducing FBG level and improving the BW to the normal state.

Keywords:

Germinated black rice , diabetes mellitus , Blood glucose , Body weight , Antioxidant