Comparison of genetic diversity and growth traits among Fangzheng silver crucian carp (Carassius auratus gibelio) gynogenetic clones
Publish place: Iranian Journal of Fisheries Sciences، Vol: 15، Issue: 1
Publish Year: 1394
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JIFRO-15-1_019
تاریخ نمایه سازی: 27 بهمن 1400
Abstract:
The silver crucian carp (Carassius auratus gibelio), a gynogenetic teleost, is a promising model for the study of evolutionary genetics in vertebrates. We identified ten gynogenetic clones (FZ-I~FZ-X) from triploid silver crucian carp, collected from Fangzheng County in Heilongjiang Province, China, using microsatellite markers. The genetic diversity of these gynogenetic clones was analyzed using ۵۲ microsatellite markers. A total of ۴۱۳ alleles were detected and the length of fragments ranged from ۹۶ to ۳۴۰ bp. The number of alleles per locus varied from ۲~۱۹ (mean=۷.۹۴۲۳). The observed heterozygosity at polymorphic loci ranged from ۰.۱۰~۱.۰۰ (mean=۰.۸۰). The average allele count per gynogenetic clone ranged from ۱.۹۴۲۳~۲.۱۹۲۳. The ratio of the polymorphic locus was from ۷۱.۱۵% (VII) to ۸۴.۶۱% (IX) per clone. The number of genotypes ranged from ۲~۱۰ per locus. Ten genotypes were observed by analyzing each of ۱۴ microsatellites. As a result, each gynogenetic clone could be accurately identified. In addition, the growth traits, including body weight, length, and height, among five gynogenetic clones were compared. There was a significant difference among gynogenetic clones. Clone FZ-V exhibited the best growth traits, with the largest body weight (۵۳.۱۷±۵.۲۴ g), length (۱۱.۳۸±۰.۳۷ cm) and height (۴.۶۹±۰.۱۸ cm). Our results provide basic data for the identification of silver crucian carp gynogenetic clones and can be used as a guide genetic breeding programs.
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