Research Article: Carbon dioxide anesthesia: A potential application to improve the air exposure duration of tilapia

The behavioral and physiological responses of tilapia to air exposure after pure saturated carbon dioxide (CO۲) anesthesia were analyzed, and a potential application of live tilapia dehydrated transport was evaluated by comparing it with the tricaine methanesulfonate (MS-۲۲۲) anesthesia and no anesthesia. Tilapia displayed about ۱ min of hyperactivity, and the induction time of anesthetized was about ۳ min during CO۲ anesthesia, both the behavioral analysis and plasma cortisol levels showed that tilapia were more sensitive and stressed to the CO۲ anesthesia than MS-۲۲۲ anesthesia. During air exposure, both anesthesia groups had good sedative effects, and the survival time of unanesthetized group, CO۲ anesthesia group and MS-۲۲۲ anesthesia group were ۳۳۱, ۴۸۹ and ۵۲۸ min respectively, and the survival time and survival rates of the anesthetized tilapia were significantly increased. Considering the efficacy, ease of use, as well as safety, the results of this study showed that CO۲ was an ideal anesthetic to improve air exposure duration of tilapia, and carbon dioxide anesthesia of tilapia potentially can be translated into an industrial short-time air exposure procedure.

Keywords:

Carbon dioxide , Anesthesia , Tricaine Methanesulfonate , Tilapia , Behavioral and physiological response , Air exposure duration