Bioinformatics evaluation of signaling pathways targeting hsa-miR-۷۶۹-۵p related to the OAS۱ in individuals with COVID۱۹

Background and Objective At the beginning of the New Year ۲۰۲۰, Coronavirus disease ۲۰۱۹ (COVID-۱۹) infection is continuing its spread across the world and a mild to moderate respiratory tract infection, but a subset of individuals with COVID-۱۹ progress to severe disease and respiratory failure. Evidence suggests that innate immunity is a significant component of the host defense against infections and particularly play role against viral infections. Virtually all Immune cells can contribute to innate immunity system by producing specific innate cytokines, in patients with severe and critical COVID-۱۹, to induce elevated and new intracellular molecular mechanisms for fighting off infections. In this study OAS۱ mostly linked to the host response to COVID-۱۹ infection, the cytokine-mediated signaling pathway and type I interferon signaling. These findings provide evidence that a locus containing OAS۱ has been identified as a risk locus for severe COVID-۱۹ in addition occurs in people of African ancestry independently of the Neanderthal haplotype. Also, host Micro-RNAs (miRNAs) bind to the COVID-۱۹ genome to effect viral infection and replication. Materials and Methods according to bioinformatics databases hsa-miR-۷۶۹-۵p was selected to study COVID-۱۹, then for finding more information we used NCBI, miRwalk, miRwalk۲, miRbase, DAVID database and KEEG pathwayFindings In this study, bioinformatics predicted that, this microRNA (hsa-miR-۷۶۹-۵p) is expected to target the OAS۱ in COVID-۱۹ .These findings provide evidence that the splice-site variant at this locus influences COVID-۱۹ outcomes by manipulating splicing of OAS۱.Conclusion the expected expression of OAS۱ and the negative regulatory function of the microRNA would be expected. MiRNA increase and then decrease the expression of its target gene. For this reason, the hsa-miR-۷۶۹-۵p is predicted should be raised; therefore, inhibiting thehsa-miR-۷۶۹-۵p, and ultimately leads in to the increase in the expression of the OAS۱ gene and activate the cytokine-mediated signaling pathway.

Keywords:

Bioinformatics , COVID۱۹ , OAS۱ , miRNA