Determining the frequency and diversity of Vibrio bacteria in water and shrimp in the pools of Hilleh site in Bushehr during the breeding period

Vibrio bacteria are one of the dangerous pathogens in shrimp farming. In this study,the diversity and frequency of pathogenic vibrios from water and shrimp of two farmsin Hilleh Bushehr site were sampled ۵ times every ۲۰ days. Water samples were prepared near the inlets and outlets of the pools and from deep water and poured into sterile bottles. Fresh shrimp samples were collected in clusters - randomly from different parts of the pools and at a temperature of ۴ ° C (next to ice) to Bacteriological laboratories were transferred. To count and identify bacteria, TCBS culture medium and bacteriological differential cultures including: oxidase reaction, fermentation of sugars, nitrate reduction test, amino acids, staining, etc. were used. The results were analyzed using SPSS software and ANOVA test. The results showed that the water and shrimp farmed in the pools were similar in terms of types of vibrios during the period. But in terms of the total number of vibrios and also the types of vibrios were significantly different from each other. The most known species in terms of frequency are: Vibrio gazogenes, Vibrio nereis, Vibrio natriegens, Vibrio alginolyticus. The data showed that Vibrio alginolyticus is the dominant species and has the highest frequency. The abundance of Vibrio can be a good indicator to evaluate the breeding management of each period.

Keywords:

Water , Shrimp , Vibriosis , Bushehr Hilla site