Inhibitory effect of one period of sub-maximal swimming Exercises in cell cycle stimulating genes(STAT۳) in the pathways leading to the tumor in Lung tissue of Wistar Rats exposed to tobacco-derived carcinogens (NNK)

It is believed that sports programs have minimal impact on pathological changes in the lung tissue exposed to smoking carcinogens. The aim of this study was to investigate the effects of twelve weeks submaximal swimming exercise on the expression of STAT۳ in lung tissue of rats following the exposure to the carcinogen NNK.Forty-six Wistar rats (with the average weight of ۱۰۵.۸۴ ± ۲۷.۹۳g) were divided into five groups: A) exercise(E), B) exercise-NNK(EN), C) NNK(N), D) control(C) and E)Vehicle group (V) group. Exercise groups completed swimming submaximal exercises for twelve weeks, five days per week (Water flow ۴-۱۰ L/min, ۲۵ ۶۰min). Rats in Vehicle group and NNK treatment group were respectively administered subcutaneous injections of distilled water and NNK (۱۲.۵mg/kg, once a week, ۱۲ weeks). ۴۸ hours after the last exercise, rats died and samples of their lung tissue were collected and stored in -۷۰ᵒ. STAT۳ expressions were measured by Real time PCR-ABI method. One-way analysis of variance and Tukey post hoc test (P≤۰.۰۵) were used for data analysis.There was a significant increase (p≤۰.۰۵) in the NNK group compared to E and E.N groups. There were Significant differences (P≤۰.۰۵) between E and EN groups with NNK group in the STAT۳ gene expression while there was no significant difference (P≤۰.۰۵) between exercise groups with the control and saline groups. The submaximal swimming exercise prevented STAT۳ gene expression in the lung tissue of rats following the exposure to the carcinogen NNK.It seems that by reducing the STAT۳ gene expression in the exercise groups, submaximal training can be used as a complementary therapy method along with other methods to prevent main factors leading to tumors.

Keywords:

Submaximal exercises , NNK , STAT۳