The Effect of four Weeks of Continuous Aerobic Training and Starvation on the Expression of Gene Pink۱ and Bnibp۳ in liver Tissue, liver Enzymes and lipid profile in Wistar Fatty Model Rats

Background and Objective: Regular exercise along with calorie intake promotes mitochondrial function by promoting healthy mitochondrial regeneration. The aim of the present study was to investigate the effect of four weeksof continuous aerobic training and starvation on the gene expression of pink۱ and bnibp۳ (liver mitophagy) in Wistar fat rats. Materials and Methods: The present study used an experimental design. Thirty ۱۸-week-old fatty, maleWistar rats with an average body weight of ۳۴۸±۲۵.۵۳ purchased from the Pasteur Institute of Iran were selected as the research sample. After one week of familiarity with the laboratory environment, these fatty animals were randomly divided into ۶ groups of ۵, control (n=۵) and experimental (n=۲۵), including control, starvation, starvation and ۳ days of exercise, starvation and ۵ days of exercise, ۳ days of exercise, ۵ days of exercise groups. Results: According to the statistical results of one-way analysis of variance, there was a significant decrease in triglyceride, cholesterol, liver enzymes ALT, AST in all groups compared to the control group. Furthermore, there was a significant increase in pink۱ and bnibp۳ gene expression in starvation group and starvation groups of ۳ and ۵ days of training compared to the control group. Conclusion: Four weeksof continuous aerobic training and starvation combined and alone were able to significantly reduce the status of blood lipids and liver enzymes in fatty model rats. Also, the starvation group and starvation groups along with exercise increased the activity of removing damaged mitochondria by increasing the activity of pink۱ and bnibp۳ genes compared to the control group.  

Keywords:

Non-alcoholic Fatty Liver Disease , Starvation , Exercise , Lipid Droplets , Mitophagy , PTEN-induced putative kinase