Investigating the Presence of tolC Efflux pump Gene inUrinary Isolates of Klebsiella Pneumoniae in Clinical Samples in the cityof Zahedan

Background and Aim : Klebsiella pneumoniae is the cause of many infectious diseases such asblood infection, wound, pneumonia and meningitis. Improper use of antibiotics and newmechanisms used by bacteria to survive have caused antibiotic resistance and made treatmentdifficult. Studies have shown that one of the most important mechanisms of antibiotic resistanceis the expression of efflux pump in bacteria. Efflux pumps are one of the most importantmechanisms of antibiotic resistance. The aim of this study was to investigate the presence of tolCefflux pump gene in infectious Klebsiella pneumoniae isolates from clinical samples in the city ofZahedan.Methods : In this study, DNA extraction was performed by Boiling method from ۹۶ Klebsiellapneumoniae urinary isolates. The presence of tolC efflux pump gene was checked by using PCRmethod.Results : The results showed the presence of tolC efflux pump gene in ۹۵.۸۳% of the isolates.Conclusion : Suppression of tolC efflux pump gene may be useful in the treatment and control ofKlebsiella pneumoniae infections.

Keywords:

Klebsiella pneumoniae , Efflux pump genes , tolC , Urinary isolates , Zahedan