A Pioneered Homemade NAG-۴SX۳-۳D Analyzer Coupled with Continuous Flow Injection Analysis New Approach for The On-Line Turbidimetric Measurements of Metronidazole in Pure and Pharmaceutical Formulations

The detection of metronidazole (MTZ) in pure and pharmaceutical formulations is now possible with a newly developed analytical technique based on turbidimetric measurement (from ۰ to ۱۸۰ degrees) utilizing a NAG-۴SX۳-۳D analyzer. The technique relied on the fact that phosphotungstic acid and metronidazole would precipitate white in an acidic medium, indicating the presence of an ion pair molecule. In order to measure turbidity, we used the attenuation of light after it collided with precipitated particles on surfaces with angles ranging from ۰ to ۱۸۰ degrees. We looked into both chemical and physical parameters. With a linear dynamic from ۰.۰۵ to ۲۰ mmol.L-۱, metronidazole has a correlation coefficient value ® of ۰.۹۹۹۳. From the step-wise dilution for the least concentration in the linear dynamic range of the calibration graph with an RSD percent lower than ۰.۵ percent, we find that the detection limit for ۳, ۳۵ mmol.L-۱ (n = ۸) metronidazole is ۲۹.۹۵۳ ng/sample. Four medicinal items have been successfully identified using this method. Standard addition and a t-test were used to compare the newly developed method of analysis to the tried-and-true methods (the HANNA equipment for turbidity measurement and the UV-spectrophotometric method). The researchers found no significant difference between the three methods at the ۹۵% confidence level.

Keywords:

Metronidazole Phosphotungstic acid Continuous flow injection analysis turbidity NAG , ۴SX۳ , ۳D analyzer