Taxonomic study of cyanoprokaryotes from medicinal plants bed with emphasis on phylogeny of complex taxa using ۱۶S rRNA marker
Publish place: Botanical Journal of Iran، Vol: 20، Issue: 2
Publish Year: 1398
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_ROST-20-2_002
تاریخ نمایه سازی: 23 مهر 1401
Abstract:
Cyanoprokaryotes are simple photosynthetic microorganisms which have an important role in the soil carbon and nitrogen cycle. The current study aimed to investigate the flora of cyanoprokaryotes from medicinal plants bed. Also phylogenetic analysis based on ۱۶S rRNA marker was performed to investigate the phylogenetic relationships between different cyanoprokaryotic taxa and evaluate the efficiency of this marker in separation of taxonomic boundaries between taxa especially in the case of complex taxa, which their relations are not well-defined. For this purpose, after collection of soil, isolation and purification of strains were performed. The cyanoprokaryotic taxa were identified morphologically and ۱۶S rRNA marker was used to approve the identifications. Phylogenetic analysis performed using Maximum Likelihood, Maximum Parsimony and Bayesian Inference. Totally, ۴۲ cyanoprokaryotic taxa were identified and Nostoc was an abundant genus in the soil of medicinal plants bed. The phylogenetic tree revealed Nostocales as a monophyletic group. Also, Wollea together with Anabaena, and Nostoc together with Desmonostoc created monophyletic groups. Results revealed that, ۱۶S rRNA is an effective phylogenetic marker in high classification rankings such as order, family and genus. However, ۱۶S rRNA could not be an effective marker in separation of close genera such as Nostoc and Desmonostoc.
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Authors
سمیه زارع زاده
PhD Student, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
حسین ریاحی
Prof., Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
زینب شریعتمداری
Assistant Prof., Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
معصومه سادات حسینی
MSc Graduate, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
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