A proposal for the unification of two cyanobacterial strains of Nostoc as the same species

The present research aimed to investigate through a polyphasic approach, the differences in morphological and genotypic features of two cyanobacterial strains isolated from paddy fields of northern parts of Iran, belonging to the family Nostocaceae (subsect. IV. I). Based on the reliable identification keys, the two strains were identified as Nostoc ellipsosporum (Desm.) Rabenh. ex Born. et Flah, and N. muscorum Ag. ex Born. et Flah. Sequencing of cloned bacterial ۱۶S rRNA fragment strongly indicated that, two Nostoc strains could not be discriminated by ۱۶S rRNA and ITS genes sequencing and the current morphological classification of both is invalid. Moreover, phylogenetic study of two Nostoc strains has demonstrated that, genetic relationships are in conflict with the morphological classification. Besides, after doing DNA-DNA reassociation experiments, it was concluded that, two Nostoc strains investigated might possibly be united into one species. Finally, two Nostoc species were named as Nostoc sp. Bahar_E and Nostoc sp. Bahar_M, and have been registered under DDBJ, accession numbers: JF۷۹۵۲۷۸ and JF۲۷۲۴۸۲, respectively.

Keywords:

DNA-DNA hybridization , ITS , Morphology , Nostoc , ۱۶S rRNA