Assessment of Neutrophil Extracellular Traps (NETs) and Macrophage Ratio in the Inner and Outer Area of Carotid Artery Stenosis

Background & Objective: It is noteworthy that vast data links NETs to human arterial thrombosis. In the current study, extracellular neutrophil networks and macrophage polarization were assessed in the area outside and inside the Carotid artery stenosis.Methods: The sample of Carotid plaque of the patient was divided into two halves with a transverse incision; the terms inner part and outer part were used for the plaque's inner part and the adjacent area. Samples were sorted in ۱۰% formalin for CD۱۶۳, CD۱۱c, MPO, and histone H۳ immunohistochemistry assessment, while part of the sample was stored at -۸۰°C for western blotting assay with PDA۴ marker.Results: Results of this study showed that the extracellular neutrophil in the inner part of the Carotid plaque was significantly increased (P<۰.۰۰۰۱), while the number of M۱ and M۲ macrophages was higher in the inner part compared with the outer part of the Carotid plaque (P<۰.۰۰۰۱).

Keywords:

Arteries , NETs , MACROPHAGE , Plaque