Research Article: Bioactive peptides from the Pacific white-leg shrimp (Litopenaeus vannamei) induce apoptosis and anticancer activities in HCT-۱۱۶ colon cancer cell line
Publish place: Iranian Journal of Fisheries Sciences، Vol: 21، Issue: 5
Publish Year: 1401
نوع سند: مقاله ژورنالی
زبان: English
View: 141
This Paper With 12 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_JIFRO-21-5_006
تاریخ نمایه سازی: 29 آذر 1401
Abstract:
In this study, the effects of white-leg shrimp hydrolysate protein extracted in a progressive rise in temperature (۴۰-۶۰°C) (Gradual) and without centrifugation (Mix) on the colon cancer cells were determined. Both bioactive peptides were prepared using enzymatic hydrolysis with alcalase. The cytotoxic effect on HCT-۱۱۶ cell line was evaluated using the Neutral red and MTT assay. In-vitro, antioxidant activity was performed using DPPH, TAC, and nitric oxide assays. Apoptosis with acridine orange/etho bromide and redox changes was evaluated in the cell lines.The results of toxicity assays showed that the survival rate of the cells were decreased by increasing the concentrations of the peptides (۰.۳, ۰.۶, ۱.۲, and ۲.۴ mg/mL). Based on the results of antioxidant activity (TAC, DPPH, No), the Gradual peptides had significantly higher antioxidant activity than Mix peptides (p<۰.۰۵). In addition, the Gradual peptide increased the concentration of nitric oxide compared to the control and Mix groups (p<۰.۰۵). Results also showed that the Gradual peptides reduced the secretion of catalase and GSH enzymes in the cancer cells (p<۰.۰۵). Both types of peptides increased apoptosis in the cancerous cell line and a higher value was observed in bioactive peptides treatments The results of the present study showed that the Pacific white shrimp hydrolysates protein obtained in a progressive rise in temperature showed anti-cancer activities against colon HCT-۱۱۶cancer cell line.
Keywords:
Authors
N. Azadi
Urmia University
E. H. Najdegerami
Urmia University
M. Imani
Urmia University
M. Nikoo
Urmia University
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :