Green Synthesis of Silver Nanoparticles using Litsea glutinosa L. Leaves and Stem Extracts and their Antibacterial Efficacy

The present study explores the green approach for the preparation of silver nanoparticles (AgNPs) through the reduction of silver nitrate by the cell-free stem and leaf aqueous extracts of Litsea glutinosa (L.glutinosa) and its potential antibacterial activity. The analytical instruments include scanning electron microscopy, Fourier transforms infrared spectroscopy, UV-visible spectroscopy, and X-ray diffraction spectroscopy confirmed the synthesis of smaller, uniformly spherical AgNPs (۱۰-۴۰ nm). The average crystalline size of prepared AgNPs produced by L. glutinosa leaf extract was found to be ۱۹ mm. From UV-visible spectral analysis, the maximum absorbance peak appeared at ۴۴۴ nm for leaf extract AgNPs different from stem extract AgNPs (۴۲۲ nm), which are found to be specific for AgNPs. The L.glutinosa stem extract-assisted AgNPs have shown significant antibacterial activity against Bacillus subtilis (Gram-positive) and Escherichia coli (Gram-negative) in comparison to Gentamycin. Hence, the AgNPs obtained by green synthesis can be therapeutically explored against bacterial infections.

Keywords:

Green synthesis , L.glutinosa , Leaf extract , AgNPs , Antibacterial activity