The Association between Preeclampsia and Defined Polymorphisms in Prothrombin and Coagulation Factor V Genes

Background & Aims: Preeclampsia is one of the complications of pregnancy and a major cause of maternal mortality. Since, hypercoagulation is one of the risk factors, defined polymorphisms of V and II coagulation factors (G۱۶۹۱A and G۲۰۲۱۰A) may increase the risk of the disease. Methods: This investigation was performed on blood samples of ۶۴ preeclamptic women and control group. DNA of white blood cells were extracted using salt satutation method. Then, G۱۶۹۱A and G۲۰۲۱۰A polymorphisms were investigated using ARMS-PCR technique. Results: Significant difference was found between the mean age of case (۲۸.۷۳۴ yrs) and control (۲۴.۹۲۱ yrs) groups (P=۰.۰۰۰۱۹۶). But, mean of gestational age did not show significant difference between the case and control groups (۳۴.۷۱۹ wks & ۳۴.۴۲۱ wks respectively).   Among the preeclamptic patients, we found two heterozygotes (۳.۱%) for each factor II and factor V. No homozygote mutation (۰%) was found in this study, while we found one heterozygote subject (۱.۶%) for factor V in the control group. Conclusion: in comparison of preeclamptic and control group for single nucleotid polymorphisms (G۱۶۹۱A and G۲۰۲۱۰A), no significant difference was found. Therefore, these polymorphisms cannot be considered as prediagnostic risk factors for preeclampsy. We suggest more wide genetic and invironmental investigations for finding preeclampsia risk factors.

Keywords:

preeclampsia , Factor V Leiden , Prothrombin