A Study on Theine and Caffeine Alkaloids' Effects on Antibiotic -resistanceBacterial Isolates

Background and purpose: nosocomial infections are increasingly resistant to current antibiotics, sothere will be serious requirements for creating new pharmaceutical forms. Coffee and tea, along withother alkaloids produced as plant metabolites and placed in the category of phytomedicine. Thisresearch, was aimed to evaluate the antimicrobial effect of alkaloid extracts from two types of coffee,including Arabica and Robusta, as well as two types of black and green tea, against drug-Resistanceisolates of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.Materials and methods: Antimicrobial susceptibility of ۲۹۷ isolates from eight hospitals in Golestanprovince was determined using the Kirby-Bauer method and according to the Clinical and LaboratoryStandards Institute document M۱۰۰-S۲۵ (۲۰۱۵). Broth microdilution susceptibility testing was carriedout to determine minimal inhibitory concentrations (MICs) of the alkaloid extracts.Results: The results indicate the positive effects of these extracts on isolate of ۳۸ MDR P.aeruginosa (n=۷۸), ۵۱ MDR E.coli (n=۹۱) and ۲۷ MDR S.aureus (n=۴۳) respectively. Also, Robustacoffee compared to Arabica coffee and green tea compared to black tea had relatively more inhibitoryeffects. Quantitative analyzes regarding the determination of the amount of caffeine and theine in theextracts also indicated a high dose of caffeine in Robusta coffee extract, which indicated the directeffects of this substance in creating antibacterial properties.Conclusion: Our findings indicate the high frequency of multi-drug resistant bacteria in hospitals.So, recommended to use caffeine (coffee) and theine (tea) to inhibit or control such resistance. Furtherresearch is required to verify the efficacy of this extracts for disinfection of hospitals.

Keywords:

Caffeine , Theine , Antibacterial effect , nosocomial infection