Frequency of CYP۱A۱ Gene Polymorphisms in Infertile Men with Non-obstructive Azoospermia

Background and Aims: The cytochrome P۴۵۰ ۱A۱ (CYP۱A۱) plays a curial role in phase I metabolism of polycyclic aromatic hydrocarbons to their ultimate biologically active intermediates that have potential reproductive toxicity in men. Reproductive functions in men may be impaired by many environmental, physiologic, and genetic factors. The majority of the environmental factors are xenobiotics. Metabolic active xenobiotics exert adverse effects via covalent interactions between intermediate metabolites and cellular macromolecules such as DNA or protein. Materials and Methods: Genotyping two polymorphisms, CYP۱A۱*۲A and CYP۱A۱*۲C, was done using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) assay in a case–control study including ۱۰۵ infertile men and۱۰۴ healthy fertile subjects. Results: The results showed that frequency of CYP۱A۱*۲A was significantly different between the patients and the controls (p = ۰.۰۳۶). Analysis indicated that CYP۱A۱*۲A CC genotype was significantly associated with an increased risk of male infertility (OR = ۵.۴, CI=۱.۱۲-۲۶.۰۴; ۹۵%) compared with the AA genotype. No significant association was detected between CYP۱A۱*۲C polymorphism and male infertility. Conclusions: The CYP۱A۱*۲A single nucleotide polymorphism can be considered as an effective agent in azoospermia.

Keywords:

Azoospermia , Cytochrome P-۴۵۰ ۱A۱ , Genotyping , Polymorphism