Molecular Detection and Characterization of Herpesvirus Papio ۲ (HVP۲) in Wild-Caught Olive Baboons from Selected Regions in Kenya
Publish place: Infection Epidemiology and Microbiology، Vol: 8، Issue: 4
Publish Year: 1401
نوع سند: مقاله ژورنالی
زبان: English
View: 85
This Paper With 12 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IEM-8-4_007
تاریخ نمایه سازی: 29 فروردین 1402
Abstract:
Aims: There is a remarkable similarity between Herpesvirus papio ۲ (HVP۲) infecting baboons and human simplex virus (HSV) in terms of molecular biology, protein functions, and resulting infections. However, no definitive therapy exists, and the available drugs only improve the clinical signs of recurrent or asymptomatic infections. This research results may be useful for studies on the quest for HVP۲ curative and preventive drugs in baboon models. Later, a similar study could be done on HSV in humans.
Materials & Methods: A total of ۶۰ baboons were sampled from six different counties in Kenya. Of these, ۵۱ cases were wild caught from five counties, and nine cases were from the Institute of Primate Research (IPR) colonies designated as captive baboons. Oral and genital swabs were collected for analysis. The trigeminal ganglia of three study subjects were also aseptically sampled. Polymerase chain reaction test was used to determine the prevalence of HVP۲. HVP۲-positive samples were sequenced and aligned to GenBank sequences using BLAST to identify specific circulating strains and generate phylogenetic relationships. DnaSP۶ was used for genetic diversity analysis.
Results: Among ۶۰ baboons studied, ۶۵% were positive for the virus. One strain, A۹۵۱, was identified as the prevalent strain. Extremely low fixation index values (Fst) were recorded, showing low genetic diversity within and between subpopulations.
Conclusion: The identified strain was non-pathogenic but could be clinically manifested as painful sores on the host's mucosal membranes and cause stillbirths. The virus prevalence was ۷۵.۸۶% in genital samples and ۵۴.۸۶% in oral samples, indicating that oral transmission is less common than genital transmission.
Keywords:
Authors
Gloria Kemunto
Biochemistry Department, Jomo Kenyatta University of Agriculture and Technology, ۶۲,۰۰۰-۰۰۲۰۰, Nairobi, Kenya
Mbogo Kevin
Biochemistry Department, Jomo Kenyatta University of Agriculture and Technology, ۶۲,۰۰۰-۰۰۲۰۰, Nairobi, Kenya
Kamau Joseph
Institute of Primate Research, P.O Box ۲۴۴۸ Karen ۰۰۵۰۲, Nairobi, Kenya.
Atunga Nyachieo
Institute of Primate Research, P.O Box ۲۴۴۸ Karen ۰۰۵۰۲, Nairobi, Kenya.
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :