Chronic stress-induced apoptosis is mitigated by young mitochondria transplantation in the prefrontal cortex of aged rats
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 26، Issue: 6
Publish Year: 1402
نوع سند: مقاله ژورنالی
زبان: English
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JR_IJBMS-26-6_015
تاریخ نمایه سازی: 21 اردیبهشت 1402
Abstract:
Objective(s): Apoptosis is common and often comorbid with aging and stress-related mood disorders. Evidence suggests that fresh mitochondria could reverse age-related dysfunctions in organs, especially in the brain. Therefore, this study investigated the effect of young mitochondria administration on the apoptosis process in the prefrontal cortex (PFC) of aged rats exposed to chronic stress. Materials and Methods: Aged (۲۲ months old) male rats were randomly assigned into four groups: aged control (AC), aged rats treated with young mitochondria (A+M), aged rats subjected to chronic stress for four weeks (A+St), and aged rats subjected to chronic stress and treated with young mitochondria (A+St+M). A+M and A+St+M groups received a single ICV injection (۱۰ μl) of fresh mitochondria isolated from the brain of young rats for five minutes (۲ µl/min). Finally, the levels of Malondialdehyde (MDA), Cytochrome c (Cyt c), Bax, Bcl-۲, and Caspase-۳ expression were investigated in the PFC.Results: Young mitochondria administration reduced neuronal apoptosis in the PFC, associated with down-regulation of MDA, Bax, and Caspase-۳ and up-regulation of Bcl-۲. Moreover, fresh mitochondria partially improved the chronic stress-induced mitochondrial dysfunction in aged rats, as indicated by reduced cytochrome c (Cyt c) release from the mitochondria.Conclusion: These results suggest mitotherapy could reverse cell viability and mitochondrial dysfunction-induced apoptosis in the PFC tissue of aged rats subjected to stressful stimuli.
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Authors
Gonja Javani
Drug Applied Research, Tabriz University of Medical Sciences, Tabriz, Iran
Arshad Ghaffari-Nasab
Drug Applied Research, Tabriz University of Medical Sciences, Tabriz, Iran
Fereshteh Farajdokht
Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Gisou Mohaddes
Drug Applied Research, Tabriz University of Medical Sciences, Tabriz, Iran
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