Role of Long non Coding RNAs, NEAT۱ and Lnc-DC Expression in Pediatric Immune Thrombocytopenic Purpura

Background: Pediatric immune thrombocytopenic purpura (ITP) is an autoimmune disease; whose etiology is unknown. lncRNAs are regulators of numerous actions, which participate in the development of autoimmune diseases. We evaluated the expression ofNEAT۱ and Lnc-RNA in dendritic cell (Lnc-DC) in pediatric ITP. Methods: Sixty ITP patients and ۶۰ healthy subjects were enrolled in the present study; Real-time PCR was performed to assess the expression levels of NEAT۱ and Lnc-DC in sera of children with ITP as well as healthy children. Results: Both lncRNAs, NEAT۱ and Lnc-DC were significantly upregulated in ITP patients in comparison to controls (p <۰.۰۰۰۱ and P= ۰.۰۰۱ respectively). Furthermore, significant upregulation of the expression levels of NEAT۱ and Lnc-DC were observed in the non-chronic compared with chronic ITP patients. Also, there was significant negative correlation between each of NEAT۱ and Lnc-DC and platelet counts before treatment (r= -۰.۳۸; P= ۰.۰۰۳ and r= -۰.۴۶۱; P< ۰.۰۰۰۱, respectively). Conclusions: serum lncRNAs, NEAT۱ and Lnc-DC could be used as potential biomarkers in differentiating childhood ITP patients and healthy controls in addition to differentiating non-chronic from chronic ITP which may provide a theoretical basis for the mechanism and treatment of immune thrombocytopenia.

Keywords:

Lnc-DC , NEAT۱ , Pediatric , ITP.