Comparison of the effect of olanzapine and pimozide on development and changes of oocytes , endocrine marker and aromatase enzyme in Trichogaster trichoptrus

In this survey the effects of olanzapine and pimozide on endocrine hormones and aromatase enzyme in Trichogaster trichopterus were analyzed. In this reason ۱۲۰ mature female fishes were divided into ۸ treatments and two control groups the fishes in one control group received ethanol ۷۰% while the other control group was intact.All injections were done  in muscles between  dorsal fin and lateral line (۰/۰۲ mL per injection). Fishes were killed ۷ days after the injection and their ovaries were dissected for histologic studies the fishes ovaries were fixed in formaline ۱۰%. After tissue passage and cutting and Hand E staining the ovary tissues in all treatment groups were studied by optical microscope and were compared with control groups. Then, endocrine hormone and aromatase levels in all treatment groups were compared with fishes in control groups by SPSS software and DUNCAN and one way of ANOVA exams.the differences between averages in different treatment fishes were determined with p-value≤۰/۰۵. The results of comparison between control and treatment groups of ۱, ۲, ۳ and ۴ receiving ۱, ۳, ۶, and ۱۰ mg/kgb.w of olanzapine relatively and treatment groups of ۵, ۶, ۷ and ۸ receiving ۱,۳,۶ and ۱۰ mg/kgb.w of pimozide relatively showed the effects of dopamine antagonists on endocrine hormones and aromatase enzyme which decreases aromatase and testosterone and estradiol levels and thus had a reasonable difference with control fishes. Studying the oocytes histologic samples by photo microscopy in control group showed that cells were in vitellogenesis and maturation stages while in pimozide and olanzapine treatments by increasing the dosages despite presence of cells in cortical stage the cell number in prenuclear stage increase steadily. The results of this study showed that dopamine antagonists effect of oocyte maturation.

Keywords:

Olanzapine , Pimozide , Endocrine hormones , aromatase , Trichogaster trichopterus