Evaluation of the effects of siRNA on Snail۱ and miR-۱۴۳ gene expression levels in metastatic female breast cancer cells during mastectomy

Introduction: The present study examines the effects of siRNA on the expression level of Snail۱ and miR-۱۴۳ genes in metastatic breast cancer cells during mastectomy and the results of this study are in the field of etiology and cellular and molecular foundations.Methodology: The synthesis of cDNA strand was done with the help of hexamer primer, which binds non-specifically at different points of the RNA strand, and as a result, all RNA strands are used as synthesis templates. For this purpose, Total RNA was first extracted from the cell culture medium for cDNA synthesis.Results: The results of knockdown evaluation of Snail۱ gene by specific siRNA showed that the knockdown of this gene in metastatic cancer cells increases the relative expression of miR-۱۴۳ in the effective dose and time compared to untreated cells.Conclusion: Transfection of breast cancer cells by specific siRNA by reducing the relative level of snail۱ gene expression and increasing the relative level of miR-۱۴۳ expression can successfully reduce proliferation and invasion. become breast cancer cells and this can play an important role in the use of cellular and molecular methods in the treatment of breast cancer.

Keywords:

siRNA , snail۱ , miR-۱۴۳ , Mastectomy