Recognition and complete investigation of the antimicrobial effect of fetal umbilical cord stem cell medium conditioning

Umbilical cord blood was once thought of as a waste product. Now, years after the first successful umbilical cord blood transplant, more families seek information about whether or not to save their newborn’s cord blood. Childbirth educators may be one of the main sources that an expectant family depends on to gain more knowledge about cord blood banking in order to make an informed decision. Cord blood (umbilical cord blood) is blood that remains in the placenta and in the attached umbilical cord after childbirth. Cord blood is collected because it contains stem cells, which can be used to treat hematopoietic and genetic disorders such as cancer. Umbilical cord blood contains blood-forming stem cells, which can renew themselves and differentiate into other types of cells. Stem cells are used in transplants for patients with cancers like leukemia and lymphoma. Cord Blood can be used to treat over ۸۰ other life- threatening diseases. Antimicrobials are generally said to the materials that are used to fight with micro-organisms to eradicate or inhibit their growth. In the strictest sense, the cell secretome (conditioned media) refers to the collection of proteins that contain a signal peptide and are processed via the endoplasmic reticulum and Golgi apparatus through the classical secretion pathway. Condition medium (condition culture medium) is secreted for proliferation and cell culture of Mesenchymal stem cells in laboratory culture medium. This compound has factors such as TGF-B, TNF, GM-SCF, KGF, PDGF, VEGF, EGF, IL-۱, IL-۶, IL-۸, BFGF, TGF-α, IGF-۱, IGF-۲, MCP-۱, HGF, Fibronectins and Collagen. Considering the presence of these factors and their role in the body, the aim of this study has been to investigate the effect of Condition Medium of Fetal umbilical cord. In this study ۵ encoded bacterial standard isolations including Escherichia Coli, Staphylococcus, Pseudomonas, Salmonella and Listeria were investigated using MBC and MIC method and a standard encoded Candida albicans yeast using Phagocytosis test in confluence of the condition Medium of fetal umbilical cord. The results of MIC and MBC showed that Condition Medium has the effect of growth inhibition in ۱۰۰% of condition medium density on Salmonella and Listeria monocytogenes but it doesn’t have any effect on growth inhibition of the rest of the bacteria. Also disc results showed the ineffectiveness the material on the growth of microorganisms except for the bacteria mentioned earlier. Phagocytosis test showed reinforcement in Phagocytosisic effect and cellular immune caused from it in adjacent samples with Condition medium cells of fetal umbilical cord. Furthermore, the macroscopic and morphologic study showed clearly that the size of Phagocytosis cells in treatment group is bigger than their size in the control group.

Keywords:

Medicine , Condition Medium , Stem Cells , Umbilical cord