An investigation of the anti-cancer effects of vitamin D on the expression of HE۴ gene and the viability of ovarian cancer cell line A۲۷۸۰S

Background and aims: Ovarian cancer is the second most common cause of death in Iran. A reduction in vitamin D production has been associated with an increased risk for ovarian cancer in many studies. Human epididymis protein ۴ (HE۴) is known as one of the most accurate tumor markers for the analysis of ovarian epithelial cancer illness or progression. The expression of the gene increases in many types of ovarian cancer. The aim of this study was to find whether vitamin D has anticancer effects on the viability of HE۴ gene expression. Methods: The human ovarian cancer cell line (A۲۷۸۰S) was cultured in an RPMI-۱۶۴۰ medium. To determine the inhibitory concentration (IC۵۰), the cells were treated with various concentrations of vitamin D and then incubated for ۲۴, ۴۸, and ۷۲ hours. The effect of drugs on the expression of HE۴ gene modification was measured and evaluated using the real-time polymerase chain reaction and a ۲-ΔΔCT method, respectively. Results: The IC۵۰ value for vitamin D was ۰.۳۵۹ μM, and the maximum death rates were observed after ۲۴ hours (۵۶.۲۰ ± ۵.۷۹). The HE۴ gene expression treated with vitamin D increased compared with the cells in the control group (Fold change > ۱). Conclusion: Vitamin D decreases the viability of A۲۷۸۰S cells, whereas the expression of the HE۴ gene is improved in cells treated with vitamin D compared to control cells, indicating that vitamin D may be unable to modify A۲۷۸۰S cells.

Keywords:

Vitamin D , Ovarian cancer , HE۴ gene , A۲۷۸۰S cell line