Evaluation of preventive effect of quercetin on doxorubicin-induced nephrotoxic rat model by [۹۹mTc]Tc-DMSA renal cortical scintigraphy and biochemical methods

Introduction: The purpose of this study was to evaluate possible preventive role of quercetin on doxorubicin (DOX) induced kidney toxicity using Tc-۹۹m Dimercaptosuccinic Acid ([۹۹mTc]Tc-DMSA) renal cortical scintigraphy and biochemical approaches.Methods: ۲۸ Male Wistar rats were separated into four groups. First group was intraperitoneally (i.p.) injected saline and regarded as the control group; second one was received ۱۸ mg/kg/i.p doxorubicin for three days at a ۲۴ h interval; the third and last group received ۱۰ mg/kg and  ۱۰۰ mg/kg quercetin for ۲۱ days and for the last ۳ days doxorubicin and quercetin were administrated together at the same time. On the ۲۲ th day of the experiment, [۹۹mTc]Tc-DMSA renal cortikal scintigraphy and biochemical parameters were measured.Results:DOX administration significantly increased blood urea nitrogen (۸۴۵%) and creatinine (۷۰۲%) levels in serum; nitric oxide (۱۵۸%), plasma tumor necrosis factor-alpha (۲۳۳%) and interleukin-۶ (۱۹۱%) levels in kidney tissue, and also reduced [۹۹mTc]Tc-DMSA uptake by ۲۹% in the kidneys as well. Pre-treatment with quercetin mitigated such alterations in all mentioned parameters. Conclusion: All data indicate that oxidative stress and inflammatory processes are involved in DOX-induced nephrotoxicity, which might be decreased by quercetin.In addition, [۹۹mTc]Tc-DMSA scintigraphic may be a good method for demonstrating doxorobucin-induced renal injury.

Keywords:

doxorubicin , Kidney , Quercetin , Nephrotoxicity , [۹۹mTc]Tc-DMSA