Frequency of p۵۳ Gene Codon ۷۲ Polymorphisms in Women with Breast Cancer in Iran

Backgrounds and Aims: P۵۳ gene is regarded important in pathogenesis of different cancers. Therefore, this study aimed to investigate the frequency of p۵۳ gene codon ۷۲ Arg/Pro polymorphism in women suffering from breast cancer. Materials and Methods: A total of ۹۰ patients with breast cancer and ۸۳ matched healthy control women participated in this case-control study. Genomic DNA was extracted from peripheral blood circulation, and codon ۷۲ polymorphism of the p۵۳ gene was examined using the amplification refractory mutation system polymerase chain reaction (ARMS-PCR) method with ۴ different primers contemporaneously. Results: A significant difference was observed between patients and controls (p˂۰.۰۵) in regard with allele frequency of the p۵۳ gene codon ۷۲ Arg/Pro. The distribution of genotypes frequencies was also proved to be significantly different between the cases and controls (p˂۰.۰۵). Conclusions: The results of the present study revealed that Pro/Pro homozygous and its Pro/Arg heterozygous genotypes of p۵۳ gene codon ۷۲ constitute breast cancer in our community. However, the complex instinct of the breast cancer and polymorphisms needs to be further studied in regard with different polymorphisms along p۵۳ and various genes in this population in order to clarify the role of codon ۷۲ in susceptibility of the breast cancer.

Keywords:

ARMS-PCR , Breast cancer , Codon ۷۲ Arg/Pro , P۵۳ , Polymorphism