The Homing of Spermatogonial Cells in the Cavities of a Novel Nanoscaffold

Background and Aims: In this lab trial, the effect of scaffold based on human serum albumin (HSA) and hydroxyapatite nanoparticles (HA NPs) on mouse spermatogonial cell line (SCL) was investigated. Materials and Methods: To synthesize HA NPs, calcium nitrate and diammonium phosphate at pH ۱۳ were gently added and heated at ۱۰۰ ºC for ۲۴ hours. Then serial concentrations of HA NPs was separately added to ۵۰۰ mg/mL of HSA, and immediately placed in the ۱۰۰ ºC water bath. Then, all scaffolds were cut, and incubated with SCL for ۶h, ۱۲h, and ۲۴h at ۳۷ ºC. Finally, the cell count was read, and homing of the cells was examined by optical microscopy. Results: It was found that the quantity of cells did not change by increase in concentration of HA NPs. On the other hand, increased incubation time led to decrease in cell count. Light microscopic observation of scaffold cavities after incubation showed the homing of spermatogonial cells. Conclusions: This promising scaffold must be more investigated in vitro and in vivo, and may be suitable for making artificial testis.

Keywords:

Human serum albumin , Hydroxyapatite , Scaffold , Spermatogonia , Testis