Frequency of FLT۳ ITD and FLT۳ TKD Mutations in Multiple Myeloma Patients (A Case Control Study)

Background and Aims: Multiple myeloma is a malignant proliferation of plasma cells derived from a single clone. The tumor, its products and the host response lead to organ damages. Some factors that are responsible in its pathogenesis are recognized. As FMS like Tyrosine Kinase ۳ receptor (FLT۳) mutation has been proved as a determining factor in leukemic patients the goal of this study was to find association of FLT۳ internal tandem duplication (ITD) and FLT۳ tyrosine kinase domain (TKD), mutations with multiple myeloma. Materials and Methods: This case-control study was conducted on ۶۰ paraffin-embedded bone marrow biopsies (۳۰ multiple myeloma and ۳۰ normal bone marrow specimens) in the pathology departments of Ghaem and Imam Reza hospitals in Mashhad. After sections preparation, DNA was extracted and two PCR reactions were set up for detection of FLT۳/ ITD and FLT۳/TKD mutations. Results: The Mean age of samples was ۶۴±۱۰ years. No FLT۳ mutations were detected in multiple myeloma patients. Conclusions: Our findings showed that FLT۳ mutations occurrence seem unusual in multiple myeloma.

Keywords:

FLT۳ , Multiple Myeloma , Polymerase chain Reaction , FLT۳ , میلوم ماتیپل , PCR