Isolation and Identification of Streptococcus pneumoniae from Admitted Patients in Tehran Hospitals in during ۲۰۱۳-۲۰۱۵

Background and Aim: Streptococcus pneumoniae is Gram-positive bacterium that causes pneumonia, septicemia, meningitis, otitis media, sinusitis, bronchitis, bacteremia and infectious processes is introduced. Due to the clinical importance of this bacterium and correct and timely diagnosis and separation of isolated samples from patients, this study were performed to identify and isolate strains of Pneumococcus from clinical samples by culture and PCR methods. Materials and Methods: In this study, ۱۷۵ various clinical samples were collected from admitted patients in Tehran hospitals during ۳ years (۲۰۱۳-۲۰۱۵),and culture and PCR methods used to isolate and identify of strains of Pneumococcus (after genome extraction and in the presence of specific primer) beside the standard strain ATCC ۴۹۶۱۹ (as control). Results: In this study, ۴۰ strains of Pneumococcus have been identified and approved by culture and PCR methods. Amplified amplicon in this study, was a ۱۶۰ bp fragment that were found in all strains of Streptococcus pneumoniae. Also, the PCR product was confirmed by sequencing. Conclusions: According to the obtained results in this study, PCR similar to culture, is a reliable method, but has been assessed relatively faster that can be used in the future to replace the old and conventional methods (biochemical tests and culture).

Keywords:

Streptococcus pneumoniae , Culture , Genome , Primer , PCR , استرپتوکوکوس پنومونیه , کشت , ژنوم , پرایمر , PCR