Evaluation of the sensitivity of the PCR technique in detecting genes omp۳۱, omp۲۵ and bcsp۳۱ in patients with brucellosis

Background and Aim: Brucellosis is still one of the common infections in developing countries and  some developed countries. Diagnosis of the human brucellosis is mainly based on blood culture and serological tests.also in addition to determine the root cause of brucellosis, the aim of this paper is susceptibility assessment of three bcsp۳۱, omp۳۱ and omp۲۵ in the diagnosis of brucellosis using PCR method among people with brucellosis. Materials and Methods: In this study, All the patients were diagnosed with positive immunological tests such as Wright and ELISA. samples of blood were cultured (BACTEC) and incubated at ۳۷°C for ۵ days and then they were cultured for ۳ days on Brucella agar. DNA was extracted from the colonies by Kit. The extracted DNA was used as template for accurate diagnosis of bacterial strains with gene-specific primers in PCR reactions bcsp۳۱, omp۳۱ and omp۲۵. Results and Conclusions: After the PCR reaction, results show that there is ۱۰۰% PCR power for diagnosis of bscp۳۱, ۹۰.۴۷% sensitivity for omp۳۱ and ۸۵.۷% sensitivity for omp۲۵ genes. Due to the high power and specificity and existence of bcsp۳۱ in human pathogenic species, bcsp۳۱ gene can be considered as more appropriate and confident target gene than omp۳۱ and omp۲۵ genes in the diagnosis of brucellosis in clinical samples using PCR method.

Keywords:

Brucellosis , PCR , Detection Power , omp۲۵ , omp۳۱ , bcsp۳۱ , بروسلوزیس , PCR , قدرت جداسازی , omp۳۱ , omp۲۵ و bcsp۳۱