Streptococcus Mutans Serotype Analysis from Dental Plaque of Caries Patients in Banda Aceh Based on the GTF Gene
Publish Year: 1402
نوع سند: مقاله ژورنالی
زبان: English
View: 76
This Paper With 6 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_RBMB-12-1_020
تاریخ نمایه سازی: 31 مرداد 1402
Abstract:
Background: Dental caries is an oral disease that is widely suffered by the population of Aceh caused by Streptococcus mutans. S. mutans serotypes c and d are widely isolated in the human oral cavity. This research was focused on detecting the presence and variability of S. mutans in supragingival dental plaque of caries teenager and young adults’ patients.
Methods: Subjects involved in this study were patients who treated at the Rumah Sakit Gigi dan Mulut of Dentistry Faculty of Universitas Syiah Kuala. The approach used in this research was molecular microbiology technique. To determine the presence of S. mutans, supragingival plaque from caries patients was cultivated in TYS۲۰B. The culture findings were utilized to detect the presence of bacteria using PCR. The primers utilized in the PCR were S. mutans specific primers, GTFB (۵۱۷ bp) for S. mutans serotype c and GTFI (۷۱۲ bp) for S. mutans serotype d.
Results: Culture results on TYS۲۰B media showed the growth of S. mutans colonies isolated from the supragingival plaque of research subjects. PCR results also revealed the presence of S. mutans in the supragingival plaques of caries patients, with the variability of S. mutans discovered to be a serotype c and a serotype d.
Conclusions: Based on the findings of this study, it can be concluded that S. mutans can be found in the supragingival plaques of caries patients with the serotypes c and d variability.
Keywords:
Authors
Santi Chismirina
Graduate School of Department of Mathematics and Science Application, Universitas Syiah Kuala, Banda Aceh, ۲۳۱۱۱, Indonesia & Faculty of Dentistry, Universitas Syiah Kuala, Banda Aceh ۲۳۱۱۱, Aceh, Indonesia.
Suzanna Sungkar
Faculty of Dentistry, Universitas Syiah Kuala, Banda Aceh ۲۳۱۱۱, Aceh, Indonesia.
Muhammad Adlim
Department of Chemistry, FKIP, Universitas Syiah Kuala, Banda Aceh ۲۳۱۱۱, Aceh, Indonesia.
Darmawi Darmawi
Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh ۲۳۱۱۱, Aceh, Indonesia.
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :