RT-qPCR Analysis of LAMP۳ (CD۲۰۸) Gene Expression in Oral Lichen Planus and Oral Squamous Cell Carcinoma

Background: Many new studies have been conducted on cellular proteins to use them as prognostic markers or in target therapy through determining the increase or decrease in their expression in the lichen planus and OSCC. LAMP۳ protein is one of these proteins which has been recently considered. Thus, considering the unknown etiology of lichen planus, significance of their early diagnosis and treatment and lack of a suitable and final treatment for this disease and oral cancers, and preventing the progression of lichen planus, which can turn into OSCC, we decided to investigate the level of expression of this gene and its effect on the progression, study the connection between these two conditions and the probable factors contributing to their etiopathogenesis. Methods: In this study, ninety-four paraffin blocks tissue samples of patients were obtained together with their demographic documents. LAMP۳ expression was measured RT-qPCR method. Results: The results show that there is not any significant difference between age and sex population of our study. in squamous cell carcinoma the amount of expression of LAMP۳ was higher than lichen planus and healthy margin. Average LAMP۳ Gene expression in grade III was higher than group grade I & II in which considering significant level of ۵%, it is statistically significant. Conclusions: According to the findings of this study, it can be concluded that the expression of the LAMP۳ gene in SCC lesions is higher than in healthy tissue. Hence, LAMP۳ gene expression can be used as a diagnostic biomarker.

Keywords:

CD۲۰۸ protein , LAMP۳ protein , oral Lichen Planus , Oral Squamous Cell Carcinoma.