Rapid Detection of COVID-۱۹ by RT-LAMP PCR Technique and its Comparison with Real-Time RT-PCR Method
Publish place: Iranian Journal of Medical Microbiology، Vol: 17، Issue: 3
Publish Year: 1402
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJMM-17-3_003
تاریخ نمایه سازی: 1 شهریور 1402
Abstract:
Background and Aim: Rapid antigen and antibody, serological tests, and RT ‑ PCR-based molecular methods are widely used to detect microorganisms worldwide. This study aimed to detect the covid-۱۹ using Isothermal nucleic acid amplification techniques.
Materials and Methods: In this study, we collected ۲۰۰ samples of nasopharynx and oropharynx from Jamaran Heart Hospital in Tehran. Covid -۱۹ was examined by LAMP-RT PCR and Real-Time RT- PCR techniques. The nasopharynx and oropharynx nucleic acids were extracted both by pishtaz RNA extraction kit. LAMP-RT-PCR was performed on direct samples, while Real-Time RT-PCR samples were tested only using RNA extraction samples. The probe-primer mixture of this kit was designed using a dual-target gene method that simultaneously targets the genomic sequences of the spike region and N nucleocapsid. Fluorescence was measured using Real-Time RT-PCR and LAMP-RT PCR.
Results: Clinical specimens (۵۶%) were positive using Real-Time RT-PCR technique, with mean Ct values less than ۳۰. Clinical samples (۵۲%) were positive for Covid-۱۹ in the RT-LAMP technique of RNA extraction samples. RT-LAMP technique indicated a sensitivity of ۹۲.۸%. Also in the RT-LAMP method without RNA extraction, the sensitivity of the technique was ۸۵.۷%.
Conclusion: The LAMP-RT PCR technique is emerging as an appropriate alternative to the Real-Time RT-PCR method. This technique has basic advantages, such as constant temperature amplification, thermal cycle elimination, faster results, and potentially greater detection capacity. Therefore, the RT-LAMP-PCR can be used as a quick and cost-effective technique that can be employed in all areas.
Keywords:
Rapid , Detection , SARS-CoV-۲ , RT-LAMP-PCR , Real-time RT-PCR , سریع , تشخیص , SARS-CoV-۲ , RT-LAMP , RT-PCR Real-Time- , مقایسه
Authors
Seyyed Mohammad Saeed Ghiasi
Department of Cardiac Anesthesiology, Baqiyatallah University of Medical Sciences, Tehran, Iran
Narges Yadollahi Movahed
Department of Laboratory Science, Qom Branch, Islamic Azad University, Qom, Iran
Davoud Esmaeili
Department of Microbiology and Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
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