Extraction and purification of phycocyanin from spirulina platensis and evaluating its antioxidant and anti- inflammatory activity

Phycocyanin is a blue pigment and water-soluble biliprotein from the spirulina platensis. It has great medical and medicinal properties and has been used as a nutritional supplements. Phycocyanin is also a natural and powerful antioxidant and anti-inflammatory. In this study, the phycocyanin of Spirulina platensis was extracted by using two enzymatic and ultrasonic methods and purified by ammonium sulfate precipitation and dialysis. The UV spectrophotometer absorption of the extracted sample showed a broad peak range at ۲۸۰, ۶۱۵, and ۶۵۲ nm. By using The FT-IR results confirmed the structure and molecular bonds from extracted and purificated phycocyanin. The concentrations obtained at the enzymatically was ۰.۴۰۵ mg/mL and for sonication ۰.۴۲۲ mg/mL. By using the SDS PAGE method, bands of phycocyanin were identified and the molecular weight was determined between ۱۹-۱۴ KD. The antioxidant and anti-inflammatory activity of the phycocyanin was approved with the reaction by DPPH and HOCl.

Keywords:

Phycocyanin , Spirulina platensis , NADPH Oxidase