The Effect of Oxidative Stress on Proteomics of Listeria monocytogenes PTCC ۱۲۹۷

Listeria monocytogenes is a ubiquitous Gram-positive food-borne human bacterial pathogen that can cause listeriosis. This disease is a fatal with a high rate of hospitalization (>۹۰%). The aim of this study is to determine the effect of hydrogen peroxide (H۲O۲) on L. monocytogenes PTCC ۱۲۹۷ proteomics. Bacterial cells exposed to gradually increasing sub-lethal concentrations of oxidative stress: ۰.۰۶, ۰.۳, ۰.۶, and ۱.۵ % of H۲O۲. Changes in protein profile of cells exposed to H۲O۲ and control (non-adapted cells) were determined by isoelectric focusing (IEF) and sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). Protein spots visualized by staining with colloidal Coomassie Brilliant Blue. Based on the obtained results, one thousand four hundred spots identified on the gels. Out of these points, ۸۵ spots were reproducibly detected with the help of software and eye confirmation. After analyzing these points, ۹ spots showed the most significant changes. These points had appropriate density and indicate the response of bacteria to stress conditions. It can be concluded that the structure of proteome of L. monocytogenes PTCC ۱۲۹۷ changes when faced with oxidative stress. Nine protein spots on the gel were found to have substantial variations after protein spot analysis. These sites show how bacteria react under stress. Some of these spots' expressions had increased, while others had decreased.

Keywords:

Listeria monocytogenes PTCC ۱۲۹۷ , Proteomics , Oxidative Stress , IEF , SDS-PAGE