Investigating the Expression Levels of Glutathione Peroxidase and Glutathione Reductase Genes in Mastectomies Women
Publish place: International journal of Advanced Biological and Biomedical Research، Vol: 11، Issue: 3
Publish Year: 1402
نوع سند: مقاله ژورنالی
زبان: English
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JR_IJABBR-11-3_001
تاریخ نمایه سازی: 9 آبان 1402
Abstract:
Introduction: Important genes that may be expressed in women candidates for mastectomy due to breast cancer are important; The expression of these genes in these women can play an important role in the treatment of these patients; The aim of this study was investigating the level of expression of glutathione peroxidase and glutathione reductase genes in mastectomies women.Procedure: After cell culture, the cell suspension was planted in ۹۶-well microplates and treated for ۲۴ hours in a CO۲ incubator at ۳۷°C. In order to synthesize cDNA, extracted RNA molecules were used with the help of Fermentaz Revert Aid TM kit, produced in the United States.Results: The concentration of ۵۰۰ micrograms of nanoparticles caused the death of more than ۶۰% of MCF ۷ cells (P< ۰.۰۰۱) while there was no significant difference in all concentrations of nanoparticles on the normal HEK۲۹۳ cell line .Conclusion: The anti-tumor effects of zinc oxide nanoparticles were shown in increasing the expression of glutathione peroxidase and glutathione reductase genes in MCF-۷ breast cancer cell line of mastectomy candidates.Introduction: Important genes that may be expressed in women candidates for mastectomy due to breast cancer are important; The expression of these genes in these women can play an important role in the treatment of these patients; The aim of this study was investigating the level of expression of glutathione peroxidase and glutathione reductase genes in mastectomies women.Procedure: After cell culture, the cell suspension was planted in ۹۶-well microplates and treated for ۲۴ hours in a CO۲ incubator at ۳۷°C. In order to synthesize cDNA, extracted RNA molecules were used with the help of Fermentaz Revert Aid TM kit, produced in the United States.Results: The concentration of ۵۰۰ micrograms of nanoparticles caused the death of more than ۶۰% of MCF ۷ cells (P< ۰.۰۰۱) while there was no significant difference in all concentrations of nanoparticles on the normal HEK۲۹۳ cell line .Conclusion: The anti-tumor effects of zinc oxide nanoparticles were shown in increasing the expression of glutathione peroxidase and glutathione reductase genes in MCF-۷ breast cancer cell line of mastectomy candidates.
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Authors
Parmiss Adyani Kalvanagh
Postgraduate Student, experimental sciences, Tabriz, Iran
Yousef Adyani Kalvanagh
Breast Surgery Fellowship, Tabriz University of Medical Sciences, Tabriz, Iran
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