Production of polyclonal antiserum against beet western yellows virus coat protein expressed in Escherichia coli
Publish place: Journal of Crop Protection، Vol: 4، Issue: 5
Publish Year: 1394
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JCP-4-5_014
تاریخ نمایه سازی: 13 آبان 1402
Abstract:
Serological methods are commonly used methods for detection of viruses. Preparation of pure viral antigens is a crucial step in production of antibodies required for serological studies. In this research the gene encoding coat protein of a Beet western yellows virus (BWYV) isolate from Iran was amplified by PCR and was ligated into a bacterial expression vector (pET۲۶b) to obtain pET-BWYV-CP clone. Escherichia coli BL۲۱ was transformed with pET-BWYV-CP and expression of the recombinant coat protein was induced by IPTG. The expressed recombinant coat proteins were purified and used as an antigen for rabbit immunization. The antiserum was able to detect recombinant coat protein in total protein extracts of induced E. coli BL۲۱ cells in western blot analysis.
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Authors
Arezu Lotfi
Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
Masoud Shams-Bakhsh
Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
Bagher Yakhchali
National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
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