Expression analyses of some Beauveria bassiana genes in response to cuticles of four different insects

Beauveria spp. as entomopathogenic fungi have extremely diverse insect host ranges.Here, a comparative transcript analysis of a Bassiasin I (BSNI), a Dipeptidyl peptidase (DPP), a Cytochrom P۴۵۰ monooxygenase (CYP۵۲), a Mitogen activated protein kinase (MAPK), and a Neuronal calcium sensor ۱ (NCS) in two isolates of Beauveria bassiana., with low and high virulence, were carried out. For this, induction media, containing isolated cuticles from four insects, and a check medium lacking any cuticular extract were prepared and isolates were allowed to grow on them. Interestingly, up-regulation of BSNI and DPP in induction media was noted in comparison to the check, following normalization with γ-ACTIN. CYP۵۲ was up-regulated in synthetic medium in comparison to cuticular extract. MAPK expression was greater in most virulent isolate once compared with the low virulent isolate. The expression of MAPK was greater in the presence of Caliptamus italicus cuticles and synthetic medium as opposed to others. NCS expression remained on changed in all media tested and can be suggested as a reference gene in transcriptomics of Beauveria spp. genes in future.

Keywords:

Bassiasin I , Dipeptidyl peptidase , Cytochrome P۴۵۰ monooxygenase , Mitogen activated protein kinase , Neuronal calcium sensor